The transfer of a liquid under dynamic conditions onto a solid surface relies on only transient wetting/adhesion under external forces. In our previous publications we have shown that thermodynamic wetting theory is unable to explain anti-adhesion phenomena under dynamic conditions where external contact and separation forces are involved. In this study we report further finding of the anti-adhesion mechanism of ink and the non-image area of the waterless offset printing plate. Our results provide evidencet that the hardness of non-image area surface reduced dramatically after contacting hydrocarbon (undecane) and ink. FTIR (ATR) detected the ink components left on the surface of the non-image area. The nano-indentation tests showed that the non-image area of the plate responded to ink and solvent treatment in different ways from other low surface energy materials such as Teflon. No softening was detected from Teflon surface after contacting ink or hydrocarbon solvent. We proposed the softening of non-image surface (the increase in elasticity) is the most likely to be the cause of the anti-adhesion mechanism of ink in waterless offset printing. The AFM images and the printing force measurements results are provided to support our findings.